🧬 Ultimate Peptide Dosage & Reconstitution Chart (2025 Edition)

Peptide dosing can be confusing — this article demystifies everything from reconstitution ratios to injection volumes. With over 20 of the most-used peptides in 2025, this guide is built for enhanced lifters, coaches, and research-driven athletes.

🔬 What Are Peptides and Why Precision Matters

Peptides are short chains of amino acids that signal specific biological responses — often targeting growth hormone release, repair mechanisms, or metabolic modulation. Because they’re extremely dose-sensitive and measured in micrograms, small miscalculations can throw off your protocol.

In bodybuilding, peptides are most often used for:

  • Growth hormone release (e.g. CJC-1295, Ipamorelin)

  • Injury recovery (e.g. BPC-157, TB-500)

  • Fat loss and recomposition (e.g. Fragment 176-191, Tesamorelin)

  • Anti-aging or sleep (e.g. Epitalon, GHK-Cu)

🧪 Reconstitution: What It Is & How to Do It

Most peptides are shipped as a dry powder (lyophilized). To use them, they must be dissolved in a liquid — usually bacteriostatic water (BAC water) — before injecting.

💧 Reconstitution Steps:

  1. Wipe vial top with alcohol

  2. Use a clean insulin syringe to draw BAC water

  3. Inject slowly into the peptide vial, along the wall

  4. Let it dissolve — swirl gently if needed

  5. Label the vial with date + volume

✅ Use BAC water — NOT sterile water unless you’re using it all immediately. BAC water contains benzyl alcohol, which helps preserve the peptide.

📊 Full Peptide Reconstitution Chart (2025)

Here’s how to accurately reconstitute and dose the most popular peptides in bodybuilding today:

Peptide Vial Size (mg) BAC Water (mL) Concentration (mcg/mL) Typical Dose (mcg) Inject Volume (mL)
BPC-157 5 2 2500 250 0.10
TB-500 5 2 2500 2000 0.80
Ipamorelin 2 1 2000 300 0.15
CJC-1295 (no DAC) 2 1 2000 100 0.05
GHRP-6 5 2 2500 150 0.06
Hexarelin 2 1 2000 200 0.10
IGF-1 LR3 1 1 1000 40 0.04
Tesamorelin 2 1 2000 1000 0.50
PT-141 10 2 5000 500 0.10
Fragment 176-191 2 1 2000 500 0.25
GHK-Cu 5 2 2500 100 0.04
Epitalon 10 2 5000 100 0.02
PEG-MGF 2 1 2000 200 0.10
Selank 5 2 2500 300 0.12
Semax 5 2 2500 300 0.12

💡 Use this formula to calculate your injection volume:
Volume (mL) = Desired Dose (mcg) ÷ Concentration (mcg/mL)

💉 Visual Syringe Guide (What 0.1 mL Looks Like)

Small doses like 0.05 mL or 0.10 mL can be hard to visualize. A 1 mL insulin syringe (usually 100 IU) helps you measure precisely.

Volume IU (on syringe)
0.05 mL 5 IU
0.10 mL 10 IU
0.25 mL 25 IU
0.50 mL 50 IU

🧪 For most peptides, 100 IU = 1 mL.

📈 Real Example: Reconstituting BPC-157

Let’s say you have a 5 mg vial of BPC-157.

  1. Add 2 mL of BAC water → your concentration is 2500 mcg/mL

  2. You want to dose 250 mcg per injection

  3. 250 ÷ 2500 = 0.10 mL

  4. Draw to the 10 IU mark on your insulin syringe

That’s it.

⚠️ Common Dosage Mistakes to Avoid

  • ❌ Using sterile water without preservatives

  • ❌ Not labeling your vial (forgetting how much water you used)

  • ❌ Drawing air bubbles or inaccurate syringe marks

  • ❌ Storing peptides at room temperature for too long

  • ❌ Thinking “more = better” — especially with IGF-1 and GHRPs

❓ Frequently Asked Questions

Q: Can I use sterile water instead of BAC?
Technically yes, but BAC water contains benzyl alcohol which preserves peptides for up to 30 days. Sterile water must be used immediately.

Q: How long does a peptide last after mixing?
If refrigerated and handled cleanly, most are stable for ~30 days.

Q: Can I preload syringes?
Yes — preload 1–2 days at a time for convenience. Don’t freeze.

Q: Where do I inject peptides?
Most are subcutaneous (belly fat, love handle region). Some like IGF-1 are injected near the muscle worked (localized application).

Q: Do I need PCT after using peptides?
Most peptides do not suppress the HPTA. Peptides like BPC-157, TB-500, CJC-1295, and GHRPs do not require PCT.